Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Ved hjelp av en Fluorescent…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Ved hjelp av en Fluorescent PCR-kapillar-gelelektroforese teknikken for å genotype CRISPR / Cas9-mediert knockout-mutanter i en high-throughput-format
Development of Leishmania Species Strains with Constitutive Expression of eGFP
Journal (Genetics)
Development of Leishmania Species Strains with Constitutive Expression of eGFP
Detection of Copy Number Alterations Using Single Cell Sequencing
Journal (Genetics)
Detection of Copy Number Alterations Using Single Cell Sequencing
 
Click here for the English version

Ved hjelp av en Fluorescent PCR-kapillar-gelelektroforese teknikken for å genotype CRISPR / Cas9-mediert knockout-mutanter i en high-throughput-format

Article DOI: 10.3791/55586-v • 08:25 min • April 8th, 2017
April 8th, 2017 •
1, 1, 1, 1
1Cancer & Stem Cell Biology Programme, Duke-NUS Medical School

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Den genotyping teknikken beskrevet her, som fluorescerende par polymerase-kjedereaksjon (PCR) for å kapillært gelelektroforese, gjør det mulig for high-throughput genotyping av nuklease-mediert knockout kloner. Det omgår begrensninger overfor andre genotype-teknikker og er mer kostnadseffektivt enn sekvenseringsmetoder.

Tags

Genetics Genome redigering knockout innsetting / sletting mutasjons high-throughput screening direkte lyse multiplexable
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter