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Journal / Biochemistry / Fluorométrie de tension-serre dans
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Fluorométrie de tension-serre dans
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Fluorométrie de tension-serre dans

Article DOI: 10.3791/55598-v • 09:24 min • May 27th, 2017
May 27th, 2017 •
1, 1,2
1Department of Pharmacology and Physiology, University of Montréal, 2Department of Physics, University of Montréal

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Cet article décrit une amélioration de la Fluorométrie conventionnelle en tension-clamp (VCF) où les acides aminés non naturels fluorescents (fUAA) sont utilisés à la place des colorants maléimidés, pour sondage des réarrangements structurels dans les canaux ioniques. La procédure comprend l'injection d'ADN ovocytaire de Xenopus, la co-injection d'ARN / fUAA et les mesures simultanées de courant et de fluorescence.

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Biochemistry acides aminés non naturels fluorescents fluorométrie tension-clamp pince à tension ovocyte coupée Anap suppression du codon d'arrêt ambre micro-injection d'ADN,
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