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Journal / Biochemistry / Fluorescenza a tensione di serraggio in
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Fluorescenza a tensione di serraggio in
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Fluorescenza a tensione di serraggio in

Article DOI: 10.3791/55598-v • 09:24 min • May 27th, 2017
May 27th, 2017 •
1, 1,2
1Department of Pharmacology and Physiology, University of Montréal, 2Department of Physics, University of Montréal

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In questo articolo si descrive un miglioramento della convenzionale fluorometria a tensione di tensione (VCF) in cui vengono utilizzati fluorocinosi aminoacidi innaturali (fUAA) anziché coloranti maleimidi, per sondare i riarrangiamenti strutturali nei canali ionici. La procedura prevede l'iniezione di DNA Xenopus oocyte, la coinjection RNA / fUAA e le misure contemporanee di corrente e fluorescenza.

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Biochimica Edizione 123 Amminoacidi fluorescenti innaturali fluorometria tensione-tensione tensione di tensione di oocita tagliata Anap soppressione del codone di stop ambra microinjection del DNA,
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