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Journal / Biochemistry / Spänningsklämma Fluorometry in
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Spänningsklämma Fluorometry in
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Spänningsklämma Fluorometry in

Article DOI: 10.3791/55598-v • 09:24 min • May 27th, 2017
May 27th, 2017 •
1, 1,2
1Department of Pharmacology and Physiology, University of Montréal, 2Department of Physics, University of Montréal

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I denna artikel beskrivs en förbättring av konventionellt spänningsklämma-fluorometri (VCF) där fluorescerande onaturliga aminosyror (fUAA) används istället för maleimidfärger för att sondra strukturella omläggningar i jonkanaler. Förfarandet innefattar Xenopus oocyt DNA-injektion, RNA / fUAA-myntutjämning och samtidiga ström- och fluorescensmätningar.

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Biochemistry Fluorescerande onaturliga aminosyror spänningsklemfluorometri öppet oocytspänningsklämma Anap Amber Stop-kodonundertryckning DNA-mikroinjektion,
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