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Journal / Biochemistry / Måling af proteinbinding til F-actin ved…
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Biochemistry

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JoVE Journal Biochemistry
Måling af proteinbinding til F-actin ved co-sedimentering
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Måling af proteinbinding til F-actin ved co-sedimentering

Article DOI: 10.3791/55613-v • 06:17 min • May 18th, 2017
May 18th, 2017 •
1, 2, 1
1Department of Cell Biology, University of Pittsburgh School of Medicine, 2Department of Biology, University of North Carolina at Chapel Hill

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Denne protokol beskriver en metode til at teste proteinets evne til at co-sedimentere med filamentous actin (F-actin) og, hvis binding observeres, for at måle affiniteten af ​​interaktionen.

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Biochemistry actin filamentous actin F-actin actin-bindende protein co-sedimentation pelleteringsassay bindende kurve
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