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Journal / Biochemistry / High Precision FRET på enkeltmolekyliveau for…
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Biochemistry

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JoVE Journal Biochemistry
High Precision FRET på enkeltmolekyliveau for biomolekylstrukturbestemmelse
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Journal (Biochemistry)
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Author Spotlight: Illuminating New Avenues for Adipose Tissue Metabolism and Disease Prevention (Video) | JoVE
Journal (Biochemistry)
Author Spotlight: Illuminating New Avenues for Adipose Tissue Metabolism and Disease Prevention (Video) | JoVE
 
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High Precision FRET på enkeltmolekyliveau for biomolekylstrukturbestemmelse

Article DOI: 10.3791/55623-v • 11:24 min • May 13th, 2017
May 13th, 2017 •
1, 2, 2, 3, 3, 2
1Department of Chemistry, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences, University of Texas Health Science Center

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En protokol til høj præcision FRET eksperimenter på det enkelte molekylniveau er præsenteret her. Derudover kan denne metode anvendes til at identificere tre konformationelle tilstande i det ligandbindende domæne af N-methyl-D-aspartat (NMDA) -receptoren. Fastlæggelse af præcise afstande er det første skridt i retning af opbygning af strukturelle modeller baseret på FRET eksperimenter.

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Biochemistry FRET single molecule interdye distance DNA fluorophore multiparameter fluorescens detektion pulseret interleaved excitation protein mærkning
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