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Neuroscience

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JoVE Journal Neuroscience
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Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

Article DOI: 10.3791/55627-v • 10:36 min • June 13th, 2017
June 13th, 2017 •
*1, *1, 1, 2, 1
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University, 2Department of Physiology, Development and Neuroscience, University of Cambridge
* These authors contributed equally

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Summary
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Whole-cell recordings from Drosophila melanogaster photoreceptors enable the measurement of spontaneous dark bumps, quantum bumps, macroscopic responses to light, and current-voltage relationships under various conditions. In combination with D. melanogaster genetic manipulation tools, this method enables the study of the ubiquitous inositol-lipid signaling pathway and its target, the TRP channel.

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Electrophysiological Method Whole-cell Voltage Clamp Recordings Drosophila Photoreceptors Light-induced Currents Single Photon Absorption Intense Light Responses Sensory Transduction Visual Transduction Ionic Mechanisms TRP Channels Advantages Of The Technique Membrane Voltage Control Signal-to-noise Ratio Specific Indicators For Optical Measurements Preparation Size Dissection Speed Metabolic Vulnerability Fly Raising Density Constant Darkness Red Light Illumination Recording Chamber Preparation
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