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JoVE Journal
Developmental Biology

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I Ovo Electroporation i Kylling Auditory Brainstem
 
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I Ovo Electroporation i Kylling Auditory Brainstem

Article DOI: 10.3791/55628-v 10:14 min June 9th, 2017
June 9th, 2017

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Summary

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Click here for the English version.

Auditoriske hjernestam neuroner af avians og pattedyr er specialiseret i hurtig neurale kodning, en grundlæggende proces for normale hørefunktioner. Disse neuroner stammer fra tydelige forstadier af embryonisk hindbrain. Vi præsenterer teknikker, der anvender elektroporering til at udtrykke gener i baghugns kerneembryoner for at studere genfunktion under auditiv udvikling.

Tags

Developmental Biology Auditory brainstem fugle udvikling, Nucleus laminaris nucleus magnocellularis plasmid
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