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Metodi per classificare i foci citoplasmatici come granuli di stress di mammiferi
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Metodi per classificare i foci citoplasmatici come granuli di stress di mammiferi

Article DOI: 10.3791/55656-v • 09:33 min • May 12th, 2017
May 12th, 2017 •
*1,2, *1,2, 1,2,3, 1,2, 1,2, 1,2,4
1Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, 2Department of Medicine, Harvard Medical School, 3Department of Histology and Embryology, Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.
* These authors contributed equally

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Granuli di stress (SG) sono strutture citoplasmatiche non membrane che si formano in cellule esposte a varie sollecitazioni. Gli SG contengono mRNA, proteine ​​legate all'RNA, piccole subunità ribosomali, fattori correlati alla traduzione e varie proteine ​​di segnalazione cellulare. Questo protocollo descrive un flusso di lavoro che utilizza diversi approcci sperimentali per rilevare, caratterizzare e quantificare SG in buona fede .

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Biologia cellulare Numero 123 granuli RNA granuli di stress mRNA repressione di traduzione immunostaining, risposta allo stress
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