Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Flash-and-Freeze: en ny teknikk for å fange…
JoVE Journal
Biology

This content is Open Access.

JoVE Journal Biology
Flash-and-Freeze
RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes
Journal (Biology)
RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes
Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
Journal (Biology)
Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
 
Click here for the English version

Flash-and-Freeze: en ny teknikk for å fange Membran Dynamics med elektronmikroskopi

Article DOI: 10.3791/55664-v • 10:01 min • May 1st, 2017
May 1st, 2017 •
*1,2, *1, 1,3
1Department of Cell Biology, Johns Hopkins School of Medicine, 2Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 3Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi har utviklet en ny metode i elektronmikroskopi, "flash-og-fryse", som muliggjør visualisering av membran dynamikk med ms tidsoppløsning. Denne teknikken kombinerer optogenetic stimulering av neuroner med høyt trykk frysing. Her viser vi prosedyrene og beskrive protokoller i detalj.

Tags

Cellular Biology utgave 123 elektronmikroskopi optogenetics synapse membran dynamikk fryse substitusjon channelrhodopsin høytrykks-frysing exocytose endocytose tidsoppløst elektronmikroskopi flash-og-fryse
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter