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Journal / Biochemistry / Glycoproteomics of the Extracellular Matrix: A…
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Biochemistry

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JoVE Journal Biochemistry
Glycoproteomics of the Extracellular Matrix
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Journal (Biochemistry)
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Journal (Biochemistry)
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Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Article DOI: 10.3791/55674-v • 14:02 min • April 21st, 2017
April 21st, 2017 •
1, 1, 1, 1, 1
1King's British Heart Foundation Centre, King's College London

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Summary
Automatic Translation

This paper describes a methodology to prepare cardiovascular tissue samples for MS analysis that allows for (1) the analysis of ECM protein composition, (2) the identification of glycosylation sites, and (3) the compositional characterization of glycan forms. This methodology can be applied, with minor modifications, to the study of the ECM in other tissues.

Tags

Glycoproteomics Extracellular Matrix Intact Glycopeptide Analysis Mass Spectrometry Cardiovascular Tissues Glycosylation Sites Glycan Forms Tissue Fibrosis Structural Matrix Glycosylation Targeted Mechanistic Studies Extraction Buffers Tissue Preparation PBS Wash Sodium Chloride Buffer
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