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JoVE Journal Neuroscience
Assay voor het meten van nucleocytoplasmisch transport in realtime binnen motor neuronachtige NSC-34 cellen
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Assay voor het meten van nucleocytoplasmisch transport in realtime binnen motor neuronachtige NSC-34 cellen

Article DOI: 10.3791/55676-v • 08:53 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1,2
1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev

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Dit protocol beschrijft een methode om de nucleocytoplasmatische transportsnelheid binnen motor neuronachtige NSC-34 cellen nauwkeurig te meten door de real-time verandering in de nucleaire invoer van een NLS-NES-GFP eiwit te kwantificeren.

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Neuroscience nucleocytoplasmisch transport shuttle GFP NSC-34 cellen C9orf72 ALS
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