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Journal / Biology / Drosophila melanogaster Malpighi tübül epiteli…
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Drosophila melanogaster Malpighi tübül epiteli optik Quantification intrasellüler pH floresan genetik olarak kodlanmış pH göstergesi
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Drosophila melanogaster Malpighi tübül epiteli optik Quantification intrasellüler pH floresan genetik olarak kodlanmış pH göstergesi

Article DOI: 10.3791/55698-v • 11:54 min • August 11th, 2017
August 11th, 2017 •
1, 1,2
1Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, 2Department of Nephrology and Hypertension, Mayo Clinic College of Medicine

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Hücresel iyon taşıma kez değerlendirilen hücre içi pH izleyerek (pHben). Genetik olarak kodlanmış pH-göstergeler (GEpHIs) olduğu gibi hücrelerde hücre içi pH optik miktar sağlar. Bu iletişim kuralı aracılığıyla hücresel ex vivo canlı görüntüleme, Drosophila melanogaster Malpighi tübüllerin pHerry ile hücre içi pH miktar detayları, sözde ratiometric genetik olarak kodlanmış pH göstergesi.

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Biyokimya sayı: 126 iyon taşıma hücre içi pH epiteli Malpighi tübüllerin Drosophila floresan muhabir genetik olarak kodlanmış pH göstergesi pH yönetmelik canlı görüntüleme pHerry
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