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Optisk kvantifisering av intracellulær pH i Drosophila melanogaster Malpighian Tubule Epithelia med fluorescerende genetisk kodet pH indikator
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Optisk kvantifisering av intracellulær pH i Drosophila melanogaster Malpighian Tubule Epithelia med fluorescerende genetisk kodet pH indikator

Article DOI: 10.3791/55698-v • 11:54 min • August 11th, 2017
August 11th, 2017 •
1, 1,2
1Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, 2Department of Nephrology and Hypertension, Mayo Clinic College of Medicine

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Mobilnettet ion transport kan ofte vurderes ved å overvåke intracellulær pH (pHjeg). Genetisk gir kodet pH-indikatorer (GEpHIs) optisk kvantifisering av intracellulær pH i intakt celler. Denne protokollen detaljer kvantifisering av intracellulær pH gjennom mobilnettet ex vivo live-avbildning av Malpighian tubules i Drosophila melanogaster med pHerry, en pseudo-ratiometric kodet genetisk pH-indikator.

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Biokjemi problemet 126 Ion transport intracellulær pH epithelia Malpighian tubuli Drosophila fluorescerende reporter genetisk kodet pH indikator pH regulering live bildebehandling pHerry
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