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JoVE Journal Developmental Biology
Registrazioni Patch-clamp intere cellule delle cellule epiteliali primarie isolate dall'epididimo
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Registrazioni Patch-clamp intere cellule delle cellule epiteliali primarie isolate dall'epididimo

Article DOI: 10.3791/55700-v • 11:52 min • August 3rd, 2017
August 3rd, 2017 •
1,2,3, 1,2,3, 1,3, 4, 1
1School of Life Science and Technology, ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

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Presentiamo un protocollo che combina l'isolamento delle cellule e la registrazione delle patch-clamp a cellule intere per misurare le proprietà elettriche delle cellule epiteliali primarie dissociate dagli epididimidi di cauda di cauda. Questo protocollo consente di indagare le proprietà funzionali delle cellule epitidimali epididimali primarie per illustrare ulteriormente il ruolo fisiologico dell'epididimo.

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Biologia dello sviluppo Numero 126 Patch-clamp intere cellule registrazione singola cellula morsetto di corrente morsetto di tensione elettrofisiologia delle cellule proprietà passive della membrana potenziale di riposo cellule epiteliali primarie cellule principali epididimide.
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