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JoVE Journal Bioengineering
Samling og sporing af mikrobiell fællesskabsudvikling inden for en Microwell Array Platform
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Samling og sporing af mikrobiell fællesskabsudvikling inden for en Microwell Array Platform

Article DOI: 10.3791/55701-v • 09:24 min • June 6th, 2017
June 6th, 2017 •
1, 2, 1, 1,3
1Biosciences Division, Oak Ridge National Laboratory, 2Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, 3Center for Nanophase Materials Sciences, Oak Ridge National Laboratory

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Udviklingen af ​​mikrobielle samfund afhænger af en kombination af faktorer, herunder miljøarkitektur, medlemskrænkelse, træk og interaktioner. Denne protokol beskriver et syntetisk, mikrofabrikeret miljø til samtidig tracking af tusinder af samfund indeholdt i femtoliter brønde, hvor nøglefaktorer såsom niche størrelse og indeslutning kan tilnærmes.

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Bioengineering microwell mikrobielle samfund succession mikrofluidics mikrofabrikation nanofabrikation lift-off celle array niche indeslutning
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