Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Detection and Visualization of DNA Damage-induced …
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Journal (Biochemistry)
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Journal (Biochemistry)
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
 

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Article DOI: 10.3791/55703-v • 13:10 min • June 9th, 2017
June 9th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Pathology, Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

Chapters

Summary
Automatic Translation

Here, it is demonstrated how the in situ Proximity Ligation Assay (PLA) can be used to detect and visualize the direct protein-protein interactions between ATM and p53 in suspension cell cultures exposed to genotoxic stress.

Tags

Proximity Ligation Assay DNA Damage Protein Complexes Suspension Cell Cultures Visualization Quantification DNA Damage Response Repair Pathways Spatial Temporal Organization Regulation Low Cell Number Protein-protein Interactions Cultured Adherent Cells Frozen Tissues Paraffin Embedded Tissues Whole Animals BV-173 Cell Line IMDM Supplements G1 Phase Cell Cycle Ammonium Methanesulfonate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter