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JoVE Journal Biochemistry
Nachweis und Visualisierung von DNA-schadeninduzierten Proteinkomplexen in Suspensionszellkulturen unter Verwendung des Proximity Ligation Assays
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Nachweis und Visualisierung von DNA-schadeninduzierten Proteinkomplexen in Suspensionszellkulturen unter Verwendung des Proximity Ligation Assays

Article DOI: 10.3791/55703-v • 13:10 min • June 9th, 2017
June 9th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Pathology, Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

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Hier wird gezeigt, wie der in situ Proximity Ligation Assay (PLA) verwendet werden kann, um die direkten Protein-Protein-Wechselwirkungen zwischen ATM und p53 in Suspensionszellkulturen, die genotoxischen Stress ausgesetzt sind, zu detektieren und zu visualisieren.

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Biochemie Ausgabe 124 DNA-Schädigung DNA-Reparatur ATM p53 Suspensionszellkultur BCR-ABL + B-Zell-akute lymphoblastische Leukämie Protein-Protein-Interaktion,
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