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Journal / Biochemistry / Fosforilasyon Saha Tespiti İçin Oligopeptide…
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Biochemistry

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JoVE Journal Biochemistry
Fosforilasyon Saha Tespiti İçin Oligopeptide Rekabet Testi
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Fosforilasyon Saha Tespiti İçin Oligopeptide Rekabet Testi

Article DOI: 10.3791/55708-v • 09:16 min • May 18th, 2017
May 18th, 2017 •
1, 1, 2, 2, 1
1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

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Peptid rekabet testleri, çeşitli moleküler ve immünolojik deneylerde yaygın olarak kullanılır. Bu makale , in vitro oligopeptitle rekabet eden kinaz testi için ayrıntılı bir yöntem ve ilgili fosforilasyon sitelerini bulmak için yararlı olabilen ilişkili validasyon prosedürlerini açıklamaktadır.

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Biyokimya Sayı 123, peptit inhibitörü fosforilasyon AMPK Nrf2 konsensüs motifi bölgeye yönelik mutagenez
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