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Journal / Cancer Research / Ex 活体人肺肿瘤切片中居民 CD8 T…
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Cancer Research

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JoVE Journal Cancer Research
Ex 活体人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像
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Ex 活体人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像

Article DOI: 10.3791/55709-v • 08:28 min • December 27th, 2017
December 27th, 2017 •
*1, *1, *1, 2,3, 4, 2,3, 1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally

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本协议描述了一种图像驻留肿瘤浸润 CD8 T 细胞标记的人肺肿瘤切片荧光耦合抗体的方法。这项技术允许 real-time 分析 CD8 T 细胞迁移使用共聚焦显微镜。

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癌症研究 问题 130 肿瘤免疫学 人 肺肿瘤 器官切片 T 细胞 迁移 荧光 显微镜 共焦
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