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Journal / Cancer Research / Ex Vivo Billeddannelse af hjemmehørende CD8…
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Cancer Research

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JoVE Journal Cancer Research
Ex Vivo Billeddannelse af hjemmehørende CD8 T-lymfocytter i menneskelige lunge Tumor skiver bruger konfokalmikroskopi
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Ex Vivo Billeddannelse af hjemmehørende CD8 T-lymfocytter i menneskelige lunge Tumor skiver bruger konfokalmikroskopi

Article DOI: 10.3791/55709-v • 08:28 min • December 27th, 2017
December 27th, 2017 •
*1, *1, *1, 2,3, 4, 2,3, 1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally

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Denne protokol beskriver en metode til image bosat tumor infiltrerer CD8 T celler mærket med fluorescently koblede antistoffer i menneskelige lunge tumor skiver. Denne teknik giver mulighed for real-time analyser af CD8 T celle migration ved hjælp af Konfokal mikroskopi.

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Kræftforskning spørgsmålet 130 Tumor immunologi menneskelige lunge tumor organotypic skiver T-celle migration fluorescens mikroskopi Konfokal
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