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Journal / Cancer Research / Ex-Vivo Bildgebung von Resident CD8-T-Lymphozyten …
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Ex-Vivo Bildgebung von Resident CD8-T-Lymphozyten in menschlichen Lungentumor Scheiben mit konfokalen Mikroskopie
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Ex-Vivo Bildgebung von Resident CD8-T-Lymphozyten in menschlichen Lungentumor Scheiben mit konfokalen Mikroskopie

Article DOI: 10.3791/55709-v • 08:28 min • December 27th, 2017
December 27th, 2017 •
*1, *1, *1, 2,3, 4, 2,3, 1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally

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Dieses Protokoll beschreibt eine Methode, um Bild resident Tumor infiltrieren CD8 T-Zellen beschriftet mit Gewebekulturen gekoppelten Antikörpern im menschlichen Lunge Tumor Scheiben. Diese Technik ermöglicht Echtzeit-Analysen von CD8 T Zelle Migration mit der konfokalen Mikroskopie.

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Cancer Research Ausgabe 130 Tumorimmunologie Mensch Lungentumor organotypischen Scheiben T-Zell Migration Fluoreszenz Mikroskopie konfokale
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