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Journal / Cancer Research / Ex Vivo Imaging dei linfociti T CD8 residente nel …
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Cancer Research

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JoVE Journal Cancer Research
Ex Vivo Imaging dei linfociti T CD8 residente nel tumore del polmone umano mediante microscopia confocale a fette
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Ex Vivo Imaging dei linfociti T CD8 residente nel tumore del polmone umano mediante microscopia confocale a fette

Article DOI: 10.3791/55709-v • 08:28 min • December 27th, 2017
December 27th, 2017 •
*1, *1, *1, 2,3, 4, 2,3, 1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally

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Questo protocollo descrive un metodo per immagine residente tumore-infiltrazione CD8 T cellule identificate con gli anticorpi fluorescente accoppiati all'interno di fette di tumore del polmone umano. Questa tecnica permette in tempo reale analisi di migrazione delle cellule T CD8 usando la microscopia confocal.

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Ricerca sul cancro problema 130 immunologia dei tumori umani tumore del polmone organotipiche fette cellule T migrazione fluorescenza microscopia confocale
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