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Journal / Cancer Research / Ex Vivo Avbildning av bosatta CD8 T-lymfocyter i…
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Cancer Research

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JoVE Journal Cancer Research
Ex Vivo Avbildning av bosatta CD8 T-lymfocyter i Human Lung tumör skivor med konfokalmikroskopi
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Ex Vivo Avbildning av bosatta CD8 T-lymfocyter i Human Lung tumör skivor med konfokalmikroskopi

Article DOI: 10.3791/55709-v • 08:28 min • December 27th, 2017
December 27th, 2017 •
*1, *1, *1, 2,3, 4, 2,3, 1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally

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Det här protokollet beskriver en metod för att bilden bosatt tumör-infiltrera CD8 T-celler märkta med fluorescently kopplat antikroppar inom human lung tumör skivor. Denna teknik tillåter realtid analyser av CD8 T cellmigration med konfokalmikroskopi.

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Cancerforskning fråga 130 tumör immunologi mänskliga lung tumör organotypic skivor T-cell migration fluorescens mikroskopi confocal
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