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JoVE Journal Developmental Biology
Differentierande kondrocyter från perifert blod härledda humana inducerade plöripotenta stamceller
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Differentierande kondrocyter från perifert blod härledda humana inducerade plöripotenta stamceller

Article DOI: 10.3791/55722-v • 07:51 min • July 18th, 2017
July 18th, 2017 •
2, 1, 3, 1
1Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University

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Vi presenterar ett protokoll för att generera en kondrogenstamning från humant perifert blod (PB) via inducerade pluripotenta stamceller (iPSCs) med hjälp av en integrationsfri metod som innefattar embryoidkropps (EB) -bildning, expansion av fibroblastiska celler och kondrogeninduktion.

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Utvecklingsbiologi utgåva 125 inducerade pluripotenta stamceller perifert blod differentiering embryoidkropp fibroblastiska celler kondrocyt
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