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JoVE Journal Bioengineering
Metaboliske Glycoengineering Sialic syre ved hjælp af N-acyl-ændret Mannosamines
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Metaboliske Glycoengineering Sialic syre ved hjælp af N-acyl-ændret Mannosamines

Article DOI: 10.3791/55746-v • 12:06 min • November 25th, 2017
November 25th, 2017 •
1,2, 3
1Max von Pettenkofer-Institut & Genzentrum, Virologie, Nationales Referenzzentrum für Retroviren, Medizinische Fakultät, LMU München, 2Institut für Laboratoriumsmedizin, klinische Chemie und Pathobiochemie, Charité - Universitätsmedizin Berlin, 3Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg

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Sialic syre er en typisk monosakkarid-enhed fundet i glycoconjugates. Det er involveret i et væld af molekylære og cellulære vekselvirkninger. Her vil vi præsentere en metode til at ændre celle overflade sialic syre udtryk ved hjælp af metaboliske glycoengineering med N- acetylmannosamine derivater.

Tags

Bioteknologi sag 129 Sialic syre polysialic syre højtryksvæskekromatografi cellekultur metaboliske glycoengineering metaboliske oligosaccharid engineering
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