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JoVE Journal
Biochemistry
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Oprensning af biotinylerede celleoverfladeproteiner fra
 
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Oprensning af biotinylerede celleoverfladeproteiner fra

Article DOI: 10.3791/55747-v 12:03 min July 23rd, 2017
July 23rd, 2017

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En centrifugeringsgradientbaseret metode med modificeret densitet blev anvendt til at isolere epithelceller fra Rhipicephalus microplus gutvæv . Overfladebundne proteiner blev biotinyleret og oprenset gennem streptavidinmagnetiske perler til anvendelse i nedstrøms applikationer.

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Biochemistry Celleoverfladeproteiner biotinylering flåter microplus tarmepitelceller
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