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Journal / Biochemistry / Biyotinile Hücre Yüzey Proteinlerinin…
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Biochemistry
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JoVE Journal Biochemistry
Biyotinile Hücre Yüzey Proteinlerinin Arındırılması
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Biyotinile Hücre Yüzey Proteinlerinin Arındırılması

Article DOI: 10.3791/55747-v • 12:03 min • July 23rd, 2017
July 23rd, 2017 •
1, 1,2, 1
1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University

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Rhipicephalus microplus bağırsak dokusundan epitel hücrelerini izole etmek için modifiye bir yoğunluk santrifüj gradyan tabanlı metodoloji kullanıldı. Yüzeye bağlı proteinler, biyotinlenmiş ve streptavidin manyetik boncuklarla arıtılmış uygulamalarda kullanılmak üzere saflaştırılmıştır.

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Biyokimya Sayı 125 Hücre yüzey proteinleri biyotinilasyon keneler mikroplak bağırsak epitel hücreleri
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