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JoVE Journal
Biology

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瞬时表达和重组蛋白的细胞定位在培养的昆虫细胞
 
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瞬时表达和重组蛋白的细胞定位在培养的昆虫细胞

Article DOI: 10.3791/55756-v 12:09 min April 20th, 2017
April 20th, 2017

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Summary

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Click here for the English version.

Nonlytic昆虫细胞表达系统没有得到充分利用进行生产,细胞运输/定位,和重组蛋白质功能分析。这里,我们描述的方法来产生在市售鳞翅目细胞系的表达载体和随后的瞬时表达。与亚细胞荧光标记蛋白烟粉虱水通道蛋白的共定位还提出。

Tags

细胞生物学,第122,异源蛋白质的表达,昆虫细胞培养物,
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