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Journal / Biochemistry / İnsan Mitral Valfinden Proteinlerin Çıkarılması…
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Biochemistry

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JoVE Journal Biochemistry
İnsan Mitral Valfinden Proteinlerin Çıkarılması İçin Optimize Edilmiş Protokol
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Journal (Biochemistry)
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İnsan Mitral Valfinden Proteinlerin Çıkarılması İçin Optimize Edilmiş Protokol

Article DOI: 10.3791/55762-v • 09:13 min • June 14th, 2017
June 14th, 2017 •
1, 2, 1, 1, 1, 1, 2,3,4
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

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İnsan mitral kapağının protein kompozisyonu halen kısmen bilinmemektedir, çünkü analizi düşük selülarite ve dolayısıyla düşük protein biyosentezi ile karmaşıktır. Bu çalışma, mitral kapak proteomunun analizi için verimli bir şekilde protein çıkarmak için bir protokol sağlar.

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Biyokimya Sayı 124 insan normal mitral kapak protein ekstraksiyonu doku homojenizasyonu protein analizi proteomik biyokimya
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