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Biochemistry

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JoVE Journal Biochemistry
Protocole optimisé pour l'extraction de protéines à partir de la soupape mitrale humaine
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Protocole optimisé pour l'extraction de protéines à partir de la soupape mitrale humaine

Article DOI: 10.3791/55762-v • 09:13 min • June 14th, 2017
June 14th, 2017 •
1, 2, 1, 1, 1, 1, 2,3,4
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

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La composition protéique de la valvule mitrale humaine est encore partiellement inconnue, car son analyse est compliquée par une faible cellularité et donc par une faible biosynthèse des protéines. Ce travail fournit un protocole pour extraire efficacement les protéines pour l'analyse du protéome de la valvule mitrale.

Tags

Biochimie Numéro 124 valvule mitrale normale humaine extraction de protéines homogénéisation tissulaire analyse protéique protéomique biochimie
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