Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Generation of Genetically Modified Mice through…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Generation of Genetically Modified Mice through the Microinjection of Oocytes
A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult
Journal (Developmental Biology)
A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
Journal (Developmental Biology)
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
 

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Article DOI: 10.3791/55765-v • 10:19 min • June 15th, 2017
June 15th, 2017 •
1, 1
1Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales

Chapters

Summary
Automatic Translation

The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.

Tags

Genetically Modified Mice Microinjection Oocytes Mouse Genesis Gene Targeting Single Guide RNA PCR Purification Kit PCR Product Binding Buffer Wash Buffer Centrifuge
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter