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JoVE Journal Developmental Biology
Generering af genetisk modificerede mus gennem mikroinjektion af oocytter
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Generering af genetisk modificerede mus gennem mikroinjektion af oocytter

Article DOI: 10.3791/55765-v • 10:19 min • June 15th, 2017
June 15th, 2017 •
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1Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales

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Mikroinjektionen af ​​musoocytter er almindeligt anvendt til både klassisk transgenese ( dvs. den tilfældige integration af transgene) og CRISPR-medieret genmålretning. Denne protokol gennemgår de seneste udviklinger inden for mikroinjektion, med særlig vægt på kvalitetskontrol og genotyping-strategier.

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Developmental Biology Mus Microinjection pronuclear cytoplasmic CRISPR transgenic genom editing oocytes
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