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JoVE Journal Genetics
Omfattende DNA-metyleringsanalyse ved bruk av en metyl-CpG-bindende domene-fangstbasert metode i kroniske lymfocytiske leukemi-pasienter
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Omfattende DNA-metyleringsanalyse ved bruk av en metyl-CpG-bindende domene-fangstbasert metode i kroniske lymfocytiske leukemi-pasienter

Article DOI: 10.3791/55773-v • 13:21 min • June 16th, 2017
June 16th, 2017 •
1, 2
1Department of Medical Genetics, Institute of Biomedicine, Gothenburg University, 2Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Gothenburg University

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Dette arbeidet beskriver en optimalisert metyl-CpG-bindingsdomene (MBD) sekvenseringsprotokoll og en beregningsrørledning for å identifisere differensielt metylerte CpG-rike regioner hos pasienter med kronisk lymfocytisk leukemi (CLL).

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Genetikk utgave 124 DNA-metylering metyl CpG DNA-bindingsdomeneprotein differensielt metylerte regioner neste generasjons sekvensering proteinkoding og ikke-kodende gener kronisk lymfocytisk leukemi
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