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JoVE Journal Genetics
Protocolos para Montagem Padrão de DNA C-Brick Usando Cpf1
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Protocolos para Montagem Padrão de DNA C-Brick Usando Cpf1

Article DOI: 10.3791/55775-v • 12:03 min • June 15th, 2017
June 15th, 2017 •
1, 1, 1
1Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

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A proteína Cpf1 associada ao CRISPR pode ser guiada por um ARN CRISPR especialmente concebido (crRAR) para escindir o DNA de cadeia dupla nos locais desejados, gerando extremidades pegajosas. Com base nessa característica, foi estabelecido um padrão de montagem de DNA (C-Brick), e um protocolo que detalha seu uso é descrito aqui.

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Genetics biologia sintética CRISPR Cpf1 C-Brick extremidades pegajosas montagem de DNA,
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