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Journal / Developmental Biology / Mittelmaßnahme Vorbereitung von
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Developmental Biology

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JoVE Journal Developmental Biology
Mittelmaßnahme Vorbereitung von
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
Journal (Developmental Biology)
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
Journal (Developmental Biology)
Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
 
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Mittelmaßnahme Vorbereitung von

Article DOI: 10.3791/55804-v • 09:16 min • May 30th, 2017
May 30th, 2017 •
1, 1, 1, 2, 1
1Biology Department, University of Massachusetts Boston, 2Department of Bioengineering, University of California, San Diego

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Ziel dieses Protokolls ist es, einen direkten und kostengünstigen Ansatz für die Sammlung von Drosophila- Embryonen im mittleren Maßstab (0,5-1 g) und die Herstellung von Proteinextrakten, die in nachgeschalteten proteomischen Anwendungen verwendet werden können, wie Affinitätsreinigungs-Massenspektrometrie (AP-MS ).

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Entwicklungsbiologie Ausgabe 123 Extrakt Lysat Protein, Fruchtfliege Embryo Proteomik Mittelmaß Reinigung Käfig
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