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JoVE Journal Genetics
Método de Implementação de Retroviral Bidirecional e Metodologia de PCR e Análise Quantitativa de Dados Workflow
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Método de Implementação de Retroviral Bidirecional e Metodologia de PCR e Análise Quantitativa de Dados Workflow

Article DOI: 10.3791/55812-v • 12:53 min • June 14th, 2017
June 14th, 2017 •
*1,2, *3, 1, 3, 4, 1,5, 6
1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)
* These authors contributed equally

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Este manuscrito descreve o procedimento experimental e análise de software para um ensaio de site de integração bidirecional que pode simultaneamente analisar DNA de junção vetor-hospedeiro a montante e a jusante. Os produtos de PCR bidirecionais podem ser usados ​​para qualquer plataforma de seqüência a jusante. Os dados resultantes são úteis para uma comparação quantitativa de alto rendimento de alvos de DNA integrados.

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Genetics Retrovirus sites de integração sequenciação de próxima geração terapia genética retroviral células-tronco cânceres código de barras celular bioinformática
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