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Overvågning af ER / SR-calciumfrigivelse med den målrettede Ca
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Overvågning af ER / SR-calciumfrigivelse med den målrettede Ca

Article DOI: 10.3791/55822-v • 12:30 min • May 19th, 2017
May 19th, 2017 •
1, 1, 1, 1
1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

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Vi præsenterer protokoller til anvendelse af vores målrettede genetisk kodede calciumindikator (GECI) CatchER + til overvågning af hurtige calciumtransienter i det endoplasmatiske / sarkoplasmiske retikulum (ER / SR) af HEK293 og skeletmuskulatur C2C12-celler ved hjælp af realtids fluorescensmikroskopi. En protokol til in situ K d måling og kalibrering diskuteres også.

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Biochemistry Calcium imaging sarcoendoplasmisk retikulum genetisk kodet calciumindikator calciumsignalering CatchER fluorescensmikroskopi
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