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JoVE Journal Biochemistry
In Vitro Polymerisering af F-actin på tidlig Endosomes
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In Vitro Polymerisering af F-actin på tidlig Endosomes

Article DOI: 10.3791/55829-v • 12:15 min • August 28th, 2017
August 28th, 2017 •
1,2, 1, 1, 1, 1
1Department of Biochemistry, University of Geneva, 2Department of Fundamental Microbiology, University of Lausanne

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Tidlige endosome funktioner afhænger af F-actin polymerisering. Her, beskriver vi en mikroskopi-baseret i vitro assay, der genopretter Nukleering og polymerisering af F-actin på tidlig endosomal membraner i reagensglas, hvilket gør dette kompleks række reaktioner medgørlige biokemiske og genetiske manipulationer.

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Biokemi sag 126 Actin endosome RAB5 cytoskeleton trafik i vitro assay
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