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Journal / Biochemistry / In Vitro Polimerizzazione di F-actina su Early…
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JoVE Journal Biochemistry
In Vitro Polimerizzazione di F-actina su Early Endosomes
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In Vitro Polimerizzazione di F-actina su Early Endosomes

Article DOI: 10.3791/55829-v • 12:15 min • August 28th, 2017
August 28th, 2017 •
1,2, 1, 1, 1, 1
1Department of Biochemistry, University of Geneva, 2Department of Fundamental Microbiology, University of Lausanne

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Funzioni di endosoma precoce dipendono dalla polimerizzazione di F-actina. Qui, descriviamo un saggio basato su microscopia in vitro che ricostituisce la nucleazione e la polimerizzazione di F-actina sulle prime membrane endosomal in provette, rendendo questa serie complessa di reazioni suscettibili di biochimica e genetica manipolazioni.

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Biochimica problema 126 actina endosoma RAB5 citoscheletro traffico test in vitro
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