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JoVE Journal Genetics
Mapeamento genômico de interações proteína-DNA com ChEC-seq em
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Mapeamento genômico de interações proteína-DNA com ChEC-seq em

Article DOI: 10.3791/55836-v • 10:43 min • June 3rd, 2017
June 3rd, 2017 •
1, 2
1Basic Sciences Division, Fred Hutchinson Cancer Research Center, 2Department of Biology, Indiana University

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Nós descrevemos a clivagem endógena da cromatina, juntamente com a sequenciação de alto rendimento (ChEC-seq), um método ortogonal de imunoprecipitação com cromatina (ChIP) para mapear os locais de ligação de proteínas genômicas com proteínas de fusão de nucleases microcócicas (MNase).

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Genetics ChEC-seq ChIP-seq transcrição genoma-amplo Mediador,
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