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Den inverterte hjertemodellen for interstitial transudatsamling fra det isolerte rottehjertet
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Den inverterte hjertemodellen for interstitial transudatsamling fra det isolerte rottehjertet

Article DOI: 10.3791/55849-v • 07:59 min • June 20th, 2017
June 20th, 2017 •
*1,2, *1,2, 2, 3, 3, 1, 2
1Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 2Department of Molecular Cardiology, Heinrich Heine University, 3Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Heinrich Heine University
* These authors contributed equally

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Denne protokollen beskriver en metode for å samle hjerteinterstitusjonsvæske fra det isolerte, perfuserte rottehjertet. For å fysisk separere interstitial transudat fra koronar venøs utløpspreparat, er Langendorff perfusert hjerte invertert, og transsittet (interstitial fluid) dannet på hjerteoverflaten oppsamles ved bruk av en myk latexhett.

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Medisin utgave 124 Langendorff perfusjon reversert hjerte rotte interstitial fluid myokardinfarkt HPLC proteomics
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