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Misura simultanea di HDAC1 e HDAC6 attività in HeLa Cells utilizzando UHPLC-MS
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Misura simultanea di HDAC1 e HDAC6 attività in HeLa Cells utilizzando UHPLC-MS

Article DOI: 10.3791/55878-v • 09:20 min • August 10th, 2017
August 10th, 2017 •
*1, *1, 1, 1
1Section des Sciences Pharmaceutiques, Universités des Geneva–Lausanne (EPGL)
* These authors contributed equally

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Il presente metodo serve a identificare isoforma-specifici inibitori dell'istone deacetilasi (HDAC) in cellule HeLa dall'analisi UHPLC-MS di substrati multipli. Questo è un metodo privo di anticorpi sviluppato per riflettere l'attività HDAC1 e HDAC6 del vita ambiente cellulare, in contrasto con le analisi senza cellula singola isoforma.

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Chimica problema 126 HDAC1 HDAC6 inibitori cell-based test UHPLC-MS cellule HeLa
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