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JoVE Journal Genetics
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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Article DOI: 10.3791/55903-v • 11:35 min • June 16th, 2017
June 16th, 2017 •
1, 1, 1
1Cell Biology and Neuroscience, University of California, Riverside

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Recent advances in the ability to genetically manipulate somatic cell lines hold great potential for basic and applied research. Here, we present two approaches for CRISPR/Cas9 generated knockout production and screening in mammalian cell lines, with and without the use of selectable markers.

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CRISPR/Cas9 Gene Knockouts Mammalian Cells Nonhomologous End Joining Homology-directed Repair Molecular Biology Cell Lines Genetic Perturbations Dot Immunoblot Colony PCR T-REx-293 Cells DMEM Media Transvection Plasmid
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