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Journal / Genetics / Immunoprecipitazione cromatinica (ChIP) nelle…
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Genetics

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JoVE Journal Genetics
Immunoprecipitazione cromatinica (ChIP) nelle linee di cellule T delle cellule
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Immunoprecipitazione cromatinica (ChIP) nelle linee di cellule T delle cellule

Article DOI: 10.3791/55907-v • 11:39 min • June 17th, 2017
June 17th, 2017 •
1, 1, 1
1Institute of Biochemistry, University of Giessen

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Questo lavoro descrive un protocollo per l'immunoprecipitazione cromatina (ChIP) usando una linea cellulare T-cellula matura. Questo protocollo è idoneo per indagare la distribuzione di marchi specifici di istoni in siti specifici di promozione o nel genoma.

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Genetica Numero 124 ChIP H3K4me1 H3K4me3 H3K27ac H3 segni dell'istonia sonicazione taglio cromatina regolazione trascrizione.
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