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Journal / Genetics / Kromatinimmunpresipitasjon (ChIP) i…
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Genetics

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JoVE Journal Genetics
Kromatinimmunpresipitasjon (ChIP) i mus-T-cellelinjer
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Kromatinimmunpresipitasjon (ChIP) i mus-T-cellelinjer

Article DOI: 10.3791/55907-v • 11:39 min • June 17th, 2017
June 17th, 2017 •
1, 1, 1
1Institute of Biochemistry, University of Giessen

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Dette arbeidet beskriver en protokoll for kromatinimmunutfelling (ChIP) ved hjelp av en moden mus-T-cellelinje. Denne protokollen er egnet til å undersøke fordelingen av spesifikke histonmerker på spesifikke promotorsteder eller genom-bred.

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Genetikk utgave 124 ChIP H3K4me1 H3K4me3 H3K27ac H3 histonmerker sonikering skjæring kromatin regulering transkripsjon.
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