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Journal / Bioengineering / En protokol til multipel gen-knockout i mus-små…
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Bioengineering

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JoVE Journal Bioengineering
En protokol til multipel gen-knockout i mus-små tarmorganoider ved anvendelse af en CRISPR-concatemer
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En protokol til multipel gen-knockout i mus-små tarmorganoider ved anvendelse af en CRISPR-concatemer

Article DOI: 10.3791/55916-v • 11:53 min • July 12th, 2017
July 12th, 2017 •
1,2, 1,2, 1, 1, 3, 1,2
1Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge, 2Department of Genetics, University of Cambridge, 3Department of Pathology, Yonsei University College of Medicine

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Denne protokol beskriver trinene til kloning af flere enkelt-guide-RNA'er i en RNA-concatemervektor, som er særlig anvendelig til at skabe multi-gen-knockouts ved hjælp af CRISPR / Cas9-teknologi. Frembringelsen af ​​dobbelt knockouts i tarmorganoider er vist som en mulig anvendelse af denne metode.

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Bioengineering CRISPR / Cas9 knockout organoid tarm elektroporation paraloguer Wnt.
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