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Journal / Biochemistry / Preparazione dei campioni per l'identificazione…
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Biochemistry

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JoVE Journal Biochemistry
Preparazione dei campioni per l'identificazione basati sulla spettrometria di massa di RNA-binding Regions
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Preparazione dei campioni per l'identificazione basati sulla spettrometria di massa di RNA-binding Regions

Article DOI: 10.3791/56004-v • 10:52 min • September 28th, 2017
September 28th, 2017 •
1,4, 1,2, 1,3, 1,3, 1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

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Descriviamo un protocollo per identificare le proteine RNA-leganti e mappare loro regioni di RNA-binding in cellule vive utilizzando photocrosslinking UV-mediati e spettrometria di massa.

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Biochimica problema 127 interazioni della proteina-RNA RNA-binding dominio regione di RNA-binding spettrometria di massa RNA non codificante reticolazione UV
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