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Biochemistry

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JoVE Journal Biochemistry
Preparação da amostra para identificação de espectrometria de massa-baseada das regiões de ligação de RNA
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Journal (Biochemistry)
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Preparação da amostra para identificação de espectrometria de massa-baseada das regiões de ligação de RNA

Article DOI: 10.3791/56004-v • 10:52 min • September 28th, 2017
September 28th, 2017 •
1,4, 1,2, 1,3, 1,3, 1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

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Descreveremos um protocolo para identificar proteínas RNA-obrigatórias e mapear suas regiões de ligação de RNA em células vivas, usando photocrosslinking UV-mediada e espectrometria de massa.

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Bioquímica edição 127 interações proteína-RNA domínio de ligação do RNA região de RNA-obrigatórias espectrometria de massa RNAs não-codificante reticulação de UV
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