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Journal / Biochemistry / Chromatin Immunoprecipitation을위한 Cross-linked…
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Biochemistry

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JoVE Journal Biochemistry
Chromatin Immunoprecipitation을위한 Cross-linked Skeletal Musculus로부터 고품질 핵을 준비하기위한 Filtration 기반 방법
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Chromatin Immunoprecipitation을위한 Cross-linked Skeletal Musculus로부터 고품질 핵을 준비하기위한 Filtration 기반 방법

Article DOI: 10.3791/56013-v • 10:04 min • July 6th, 2017
July 6th, 2017 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston

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우리는 cross-linked 마우스 골격근으로부터 고품질의 핵을 분리하기위한 여과 기반의 프로토콜을 제시했습니다. 우리는 초 원심 분리의 필요성을 제거하여 쉽게 적용 할 수있게했습니다. 우리는 핵에서 준비한 염색질이 염색질 면역 침전 및 염색질 면역 침전 시퀀싱 연구에 적합 함을 보여줍니다.

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생화학 이슈 125 골격근 가교 결합 핵 분리 염색질 면역 침전 순차 여과 circadian time
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